diff --git a/arch/arm/boot/dts/at91-sama5d27_som1.dtsi b/arch/arm/boot/dts/at91-sama5d27_som1.dtsi
index b48ac3b62a31..a4623cc67cc1 100644
--- a/arch/arm/boot/dts/at91-sama5d27_som1.dtsi
+++ b/arch/arm/boot/dts/at91-sama5d27_som1.dtsi
@@ -8,6 +8,7 @@
  */
 #include "sama5d2.dtsi"
 #include "sama5d2-pinfunc.h"
+#include <dt-bindings/gpio/gpio.h>
 
 / {
 	model = "Atmel SAMA5D27 SoM1";
@@ -95,8 +96,11 @@ ethernet-phy@7 {
 
 			i2c0: i2c@f8028000 {
 				dmas = <0>, <0>;
-				pinctrl-names = "default";
+				pinctrl-names = "default", "gpio";
 				pinctrl-0 = <&pinctrl_i2c0_default>;
+				pinctrl-1 = <&pinctrl_i2c0_gpio>;
+				sda-gpios = <&pioA PIN_PD21 GPIO_ACTIVE_HIGH>;
+				scl-gpios = <&pioA PIN_PD22 (GPIO_ACTIVE_HIGH | GPIO_OPEN_DRAIN)>;
 				status = "okay";
 
 				at24@50 {
@@ -113,6 +117,12 @@ pinctrl_i2c0_default: i2c0_default {
 					bias-disable;
 				};
 
+				pinctrl_i2c0_gpio: i2c0_gpio {
+					pinmux = <PIN_PD21__GPIO>,
+						 <PIN_PD22__GPIO>;
+					bias-disable;
+				};
+
 				pinctrl_qspi1_default: qspi1_default {
 					sck_cs {
 						pinmux = <PIN_PB5__QSPI1_SCK>,
diff --git a/arch/arm/boot/dts/at91-sama5d27_som1_ek.dts b/arch/arm/boot/dts/at91-sama5d27_som1_ek.dts
index 614999dcb990..0f10e05d6e23 100644
--- a/arch/arm/boot/dts/at91-sama5d27_som1_ek.dts
+++ b/arch/arm/boot/dts/at91-sama5d27_som1_ek.dts
@@ -131,8 +131,11 @@ i2c3: i2c@600 {
 					i2c-analog-filter;
 					i2c-digital-filter;
 					i2c-digital-filter-width-ns = <35>;
-					pinctrl-names = "default";
+					pinctrl-names = "default", "gpio";
 					pinctrl-0 = <&pinctrl_mikrobus_i2c>;
+					pinctrl-1 = <&pinctrl_i2c3_gpio>;
+					sda-gpios = <&pioA PIN_PA24 GPIO_ACTIVE_HIGH>;
+					scl-gpios = <&pioA PIN_PA23 (GPIO_ACTIVE_HIGH | GPIO_OPEN_DRAIN)>;
 					status = "okay";
 				};
 			};
@@ -216,8 +219,11 @@ i2c1: i2c@fc028000 {
 				i2c-analog-filter;
 				i2c-digital-filter;
 				i2c-digital-filter-width-ns = <35>;
-				pinctrl-names = "default";
+				pinctrl-names = "default", "gpio";
 				pinctrl-0 = <&pinctrl_i2c1_default>;
+				pinctrl-1 = <&pinctrl_i2c1_gpio>;
+				sda-gpios = <&pioA PIN_PD4 GPIO_ACTIVE_HIGH>;
+				scl-gpios = <&pioA PIN_PD5 (GPIO_ACTIVE_HIGH | GPIO_OPEN_DRAIN)>;
 				status = "okay";
 			};
 
@@ -253,6 +259,13 @@ pinctrl_i2c1_default: i2c1_default {
 					bias-disable;
 				};
 
+				pinctrl_i2c1_gpio: i2c1_gpio {
+                                        pinmux = <PIN_PD4__GPIO>,
+                                                 <PIN_PD5__GPIO>;
+                                        bias-disable;
+                                };
+
+
 				pinctrl_isc_base: isc_base {
 					pinmux = <PIN_PC21__ISC_PCK>,
 						 <PIN_PC22__ISC_VSYNC>,
@@ -442,6 +455,12 @@ pinctrl_mikrobus_i2c: mikrobus1_i2c {
 					bias-disable;
 				};
 
+				pinctrl_i2c3_gpio: i2c3_gpio {
+					pinmux = <PIN_PA24__GPIO>,
+						 <PIN_PA23__GPIO>;
+					bias-disable;
+				};
+
 				pinctrl_flx4_default: flx4_uart_default {
 					pinmux = <PIN_PC28__FLEXCOM4_IO0>,
 						 <PIN_PC29__FLEXCOM4_IO1>,